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ywlE [2020-08-04 09:31:44]
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ywlE [2020-08-04 09:31:44]

protein arginine phosphatase
locus
BSU_36930
pI
6.68
mw
16.64 kDa
protein length
150 aa Sequence Blast
gene length
453 bp Sequence Blast
function
dephosphorylation of [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|McsB]
product
protein arginine phosphatase
essential
no
ec
3.1.3.48
synonyms
ipc-31d
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases]

    • Gene

    Coordinates
    3,791,805 3,792,257

    Phenotypes of a mutant

  • impaired spore germination [pubmed|31221751]

    • The protein

    Catalyzed reaction/ biological activity

  • Protein arginine phosphate + H2O --> protein arginine + phosphate [Pubmed|22517742]

    • Protein family

  • low molecular weight phosphotyrosine protein phosphatase family (with [protein|DB3527FB78D0B7C144D1699D665788184F19AFE0|ArsC] and [protein|2CEFD8D9692D062CC09E3DEDA1A4015C76BC4167|YfkJ], according to UniProt)

    • Paralogous protein(s)

  • [protein|2CEFD8D9692D062CC09E3DEDA1A4015C76BC4167|YfkJ]

    • Effectors of protein activity

  • activity is inhibited by oxidative stress [Pubmed|27524296]

    • Structure

  • [PDB|1ZGG]

    • [SW|Localization]

  • weak oligomerization, results in inactivation of YwlE [Pubmed|19678837]

    • Expression and Regulation

    Operons

    genes
    [gene|19829F6D993188BE854F0F61CB93777AD04CF754|ywlD]-[gene|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE]-[gene|91827FEC70F5EEB8B072D06ABA05C19FC54D5F71|ywlF]-[gene|41407DE15D91959DA92799623F1364811C170F35|ywlG]
    description
    [Pubmed|22383849]
    view in new tab

    genes
    [gene|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE]-[gene|91827FEC70F5EEB8B072D06ABA05C19FC54D5F71|ywlF]-[gene|41407DE15D91959DA92799623F1364811C170F35|ywlG]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-A186 (ywlE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/186 NBRP B. subtilis, Japan]
  • ''ywlE::aphA3'' available from [SW|Ulf Gerth]'s lab
  • ''ywlE::tet'' available from [SW|Ulf Gerth]'s lab
  • ''ywlE::spec'' available from [SW|Ulf Gerth]'s lab
  • GP1458 (''ywlE''::''aphA3''), available in [SW|Jörg Stülke]'s lab
  • GP1459 (''ywlE''::''tet''), available in [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs [Pubmed|25610436]
  • BKE36930 ([gene|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAGTCACCCCTTATT, downstream forward: _UP4_TAAGTTGTCAGAAAATCTGC
  • BKK36930 ([gene|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAGTCACCCCTTATT, downstream forward: _UP4_TAAGTTGTCAGAAAATCTGC

    • Expression vectors

  • for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [SW|Ulf Gerth]'s lab
  • expression of native ''ywlE'' in ''B. subtilis'': pBP183 (in [SW|pBQ200]), available in [SW|Fabian Commichau]'s lab

    • Antibody

  • available in [SW|Ulf Gerth]'s lab

    • labs

  • [SW|Ulf Gerth], Greifswald, Germany
  • [SW|Fabian Commichau] Göttingen, Germany [http://www.uni-goettingen.de/de/413008.html homepage]

    • References

    Reviews

  • 27541193,28748186

    • Original Publications

  • 16452434,15866923,15995210,19678837,21622759,20852588,22517742,23770242,23838530,24263382,24825175,25610436,27524296,31221751